ABSTRACT
Objective: To analyze,identify and compare the volatile components in different medicinal parts of Murrayae Folium et Cacumen(Kamuning). Methods: The volatile components were extracted by headspace solid-phase microextraction(HS-SPME)and analyzed by gas chromatography-mass spectrometry(GC-MS). The relative contents were calculated for each of the volatile components by the area normalization method. Results A total of 74 compounds were identified in the root,stem and leaf of Murrayae Folium et Cacumen,among which there were 8 components in common in the three parts,and the numbers of specific components in roots,stems and leaves were 23,27 and 32,respectively. Conclu- sion The present HS-SPME-GC-MS method was suitable for the analysis of volatile components in the different parts of Murrayae Folium et Cacumen. Some volatile components were identified to be medicinal ingredients. The present results provide a reference for further development and application of the Murrayae Folium et Cacumen resources.
ABSTRACT
Objective: To analyze the volatile components in different brands of moxa cones and sticks. Methods: Head- space solid-phase microextraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS)was used to qualitatively analyze volatile components in different brands of moxa cones and sticks. The relative content of each component was calculated by peak area normalization method. The experimental data were comprehensively evaluated by principal component analysis. Results: The total ion chromatograms of the volatile components of moxa cones and sticks in 7 batches produced by 5 manufacturers were simi- lar on the whole,but the volatile components were still different. Eighty-six compounds were identified initially. The most common components were found to be eucalyptol,camphor,terpineol,bornyl acetate,caryophyllene,caryophyllene,oxide(-)-4-terpineol, and cliff ketone. Conclusion: Using HS-SPME-GC-MS could quickly provide information for the chemical composition of the volatile componentsin themoxaconesandsticks,andtherearesignificantdifferencesin thevolatilecomponentsamongdifferentbrands.
ABSTRACT
To further study the safety and effect of the umbilical cord blood [UCB]-derived mesenchymal stem cells [MSCs] on apoptosis of human cardiac myocyte [HCM]. The UCB was collected at the time of delivery with informed consent obtained from 10 donors. The UCB-derived MSCs was treated with 5-azaserine [5-AZA], and further introduced differentiation into cardiomyocytes. The telomerase activity, G-banding patterns of chromosomal karyotypes, tumor formation in nude mice, reverse transcription polymerase chain reaction [RT-PCR], and the inhibited apoptosis of UCB-derived MSCs were further investigated. This study was carried out in the laboratory of Beijing Shijitan Hospital, Beijing, China and Inheritance Research Section of Chinese Medical Institute, Beijing, China from July 2005 to December 2007. The MSCs-derived from UCB were differentiated into cardiomyocytes in vitro, possessed telomerase activity after 5-AZA induction, and no abnormal chromosomal karyotypes were observed. Expression of p53, cyclinA, cdk2, beta-actin, C-fos, h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment. There was no tumor formation injected into nude mice. The UCB-derived MSCs significantly inhibited apoptosis of human cardiomyocytes. Umbilical cord blood-derived MSCs are safe and effective source of cell-transplantation treatment, and can inhibit the apoptosis of human cardiomyocytes in co-cultured